Can't delete iso file

[Andrei Bacalso]

Today I was downloading a iso file to be use in a virtual machine for a test, and once I was done I just deleted it but I couldn't,it said "The action cannot be completed because the file is open in System" when I just ejected it a moment ago, now when I try to mount it it just said "the disk isn't initialized" and I have to "Use the disk management snap-in to make sure the disk are in a usable state" can anyone fix it? thank you.

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